RNA SEQUENCING

We use Next Generation Sequencing technologies that allow the analysis of total RNA (WTS), but also the specific analysis of coding RNA (RNA-seq) or microRNA (miRNA-seq).

SAMPLE PREPARATION

EXTRACTION
Extraction from the different matrices (eg blood, plasma, paraffin, fresh tissue and saliva) with kits optimized for the NGS analysis required.

QUALITY CHECK
We use the main tools for checking the quality and quantity of the extracted DNA, such as NanoDrop, LabChip and Qubit.

SEQUENCING

The library preparation protocol and the type of sequencing strictly depend on the purpose of the investigation.

RNA-seq WTS miRNA-seq
Types of isolated RNA Mostly coding (with poly-A tail) Coding and not coding microRNA
Selection method poly-A selection rRNA depletion Selection of mature miRNAs (3 ‘OH, 5’ P)
Sequencing library 75SE / 75PE / 150PE 75SE / 75PE / 150PE 75SE

BIOINFORMATIC ANALYSIS

I level bioinformatic analysis II level bioinformatic analysis III level bioinformatic analysis
Demultiplexing
Production of FastQ files
Alignment on reference
Known genes expression analysis
Isoforms expression analysis

* Upon request, we can develop custom pipelines, which may include different and / or additional analysis to those in the table.

Want to know more details about our RNA sequencing service?

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