We use Next Generation Sequencing protocols that allow the analysis of microbial communities by sequencing a gene of your choice (16S or ITS amplicon sequencing) or the whole genome or transcriptome (sequencing of the entire microbial genome or shotgun sequencing of the microbial community).
DNA extraction from various biological samples with kits optimized for the required NGS analysis.
We at Personal Genomics use the main tools for verifying the quality and quantity of extracted DNA, such as NanoDrop, LabChip and Qubit.
AMPLICON SEQUENCING (16S or ITS)
The sequencing of 16S rRNA and the Internal Transcribed Spacer (ITS) are the main applications of amplicon sequencing in metagenomics.
This type of sequencing allows to identify and compare the bacterial or fungal species present in a sample.
SHOTGUN SEQUENCING OF THE MICROBIAL COMMUNITY
Shotgun sequencing of the microbial community allows you to analyze the entire genome or transcriptome of the entire community of microorganisms (metagenome / metatrascriptome) contained in a sample.
SEQUENCING AND ASSEMBLY OF THE MICROBIAL GENOME
Shotgun sequencing of the entire bacterial genome and de novo assembly.
Do you want to know more details about our metagenomics service?