The type of sequencing depends on the length of the library and the choice of capture kit. We mainly use Illumina sequencers, but we also have sequencing experience on other platforms, which can be used upon specific customer request.
EXOME (WES) AND PANELS
Selection of capture kits
We constantly evaluate the performance of new enrichment platforms. This allows us to have a proprietary database, which can be queried to evaluate which kit is most suitable for each region of the exome, from which we obtained the system for selecting the capture kit and fragment length present in the patent.
FULL GENOME (WGS)
We use PCR-free libraries to ensure the maximum possible coverage. Genomic libraries produced with protocols that involve the use of PCR, in fact, show a more irregular or absent coverage, which for us at Personal Genomics is not enough.