RNA SEQUENCING
We use Next Generation Sequencing technologies that allow the analysis of total RNA (WTS), but also the specific analysis of coding RNA (RNA-seq) or microRNA (miRNA-seq).
SAMPLE PREPARATION
EXTRACTION
Extraction from the different matrices (eg blood, plasma, paraffin, fresh tissue and saliva) with kits optimized for the NGS analysis required.
QUALITY CHECK
We use the main tools for checking the quality and quantity of the extracted DNA, such as NanoDrop, LabChip and Qubit.
SEQUENCING
The library preparation protocol and the type of sequencing strictly depend on the purpose of the investigation.
| RNA-seq | WTS | miRNA-seq | |
|---|---|---|---|
| Types of isolated RNA | Mostly coding (with poly-A tail) | Coding and not coding | microRNA |
| Selection method | poly-A selection | rRNA depletion | Selection of mature miRNAs (3 ‘OH, 5’ P) |
| Sequencing library | 75SE / 75PE / 150PE | 75SE / 75PE / 150PE | 75SE |
BIOINFORMATIC ANALYSIS
| I level bioinformatic analysis | II level bioinformatic analysis | III level bioinformatic analysis | |
| Demultiplexing | |||
| Production of FastQ files | |||
| Alignment on reference | |||
| Known genes expression analysis | |||
| Isoforms expression analysis |
* Upon request, we can develop custom pipelines, which may include different and / or additional analysis to those in the table.
