{"id":1110,"date":"2021-04-23T15:43:58","date_gmt":"2021-04-23T13:43:58","guid":{"rendered":"https:\/\/www.personalgenomics.it\/en\/?page_id=1110"},"modified":"2022-07-21T16:45:25","modified_gmt":"2022-07-21T14:45:25","slug":"dna-sequencing","status":"publish","type":"page","link":"https:\/\/www.personalgenomics.it\/en\/ngs-custom-services\/dna-sequencing\/","title":{"rendered":"DNA sequencing"},"content":{"rendered":"<p><div class=\"fusion-fullwidth fullwidth-box fusion-builder-row-1 fusion-flex-container fusion-parallax-none nonhundred-percent-fullwidth non-hundred-percent-height-scrolling\" style=\"background-color: #f0f0f0;background-image: url(&quot;https:\/\/www.personalgenomics.it\/en\/wp-content\/uploads\/sites\/2\/2021\/04\/banner-laboratorio.png&quot;);background-position: center top;background-repeat: no-repeat;border-width: 0px 0px 0px 0px;border-color:#eae9e9;border-style:solid;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;\" ><div class=\"fusion-builder-row fusion-row fusion-flex-align-items-flex-start\" style=\"max-width:1352px;margin-left: calc(-4% \/ 2 );margin-right: calc(-4% \/ 2 );\"><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-0 fusion_builder_column_1_1 1_1 fusion-flex-column\"><div class=\"fusion-column-wrapper fusion-flex-justify-content-flex-start fusion-content-layout-column\" style=\"background-position:left top;background-repeat:no-repeat;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;padding: 0px 0px 0px 0px;\"><\/div><style type=\"text\/css\">.fusion-body .fusion-builder-column-0{width:100% !important;margin-top : 0px;margin-bottom : 0px;}.fusion-builder-column-0 > .fusion-column-wrapper {padding-top : 0px !important;padding-right : 0px !important;margin-right : 1.92%;padding-bottom : 0px !important;padding-left : 0px !important;margin-left : 1.92%;}@media only screen and (max-width:1024px) {.fusion-body .fusion-builder-column-0{width:100% !important;order : 0;}.fusion-builder-column-0 > .fusion-column-wrapper {margin-right : 1.92%;margin-left : 1.92%;}}@media only screen and (max-width:640px) {.fusion-body .fusion-builder-column-0{width:100% !important;order : 0;}.fusion-builder-column-0 > .fusion-column-wrapper {margin-right : 1.92%;margin-left : 1.92%;}}<\/style><\/div><\/div><style type=\"text\/css\">.fusion-body .fusion-flex-container.fusion-builder-row-1{ padding-top : 200px;margin-top : 0px;padding-right : 0px;padding-bottom : 150px;margin-bottom : 0px;padding-left : 0px;}<\/style><\/div><div class=\"fusion-fullwidth fullwidth-box fusion-builder-row-2 fusion-flex-container nonhundred-percent-fullwidth non-hundred-percent-height-scrolling\" style=\"background-color: rgba(255,255,255,0);background-position: center center;background-repeat: no-repeat;border-width: 0px 0px 0px 0px;border-color:#eae9e9;border-style:solid;\" ><div class=\"fusion-builder-row fusion-row fusion-flex-align-items-flex-start\" style=\"max-width:1352px;margin-left: calc(-4% \/ 2 );margin-right: calc(-4% \/ 2 );\"><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-1 fusion_builder_column_1_1 1_1 fusion-flex-column fusion-animated\" data-animationType=\"fadeInDown\" data-animationDuration=\"0.3\" data-animationOffset=\"top-into-view\"><div class=\"fusion-column-wrapper fusion-flex-justify-content-flex-start fusion-content-layout-column\" style=\"background-position:left top;background-repeat:no-repeat;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;padding: 0px 0px 0px 0px;\"><style type=\"text\/css\">@media only screen and (max-width:1024px) {.fusion-title.fusion-title-1{margin-top:0px!important; margin-right:0px!important;margin-bottom:15px!important;margin-left:0px!important;}}@media only screen and (max-width:640px) {.fusion-title.fusion-title-1{margin-top:0px!important; margin-right:0px!important;margin-bottom:20px!important; margin-left:0px!important;}}<\/style><div class=\"fusion-title title fusion-title-1 fusion-sep-none fusion-title-center fusion-title-text fusion-title-size-two\" style=\"margin-top:0px;margin-right:0px;margin-bottom:15px;margin-left:0px;\"><h2 class=\"title-heading-center\" style=\"margin:0;text-transform:uppercase;\">DNA sequencing<\/h2><\/div><style type=\"text\/css\">@media only screen and (max-width:1024px) {.fusion-title.fusion-title-2{margin-top:0px!important; margin-right:0px!important;margin-bottom:15px!important;margin-left:0px!important;}}@media only screen and (max-width:640px) {.fusion-title.fusion-title-2{margin-top:0px!important; margin-right:0px!important;margin-bottom:20px!important; margin-left:0px!important;}}<\/style><div class=\"fusion-title title fusion-title-2 fusion-sep-none fusion-title-center fusion-title-text fusion-title-size-four\" style=\"margin-top:0px;margin-right:0px;margin-bottom:15px;margin-left:0px;\"><h4 class=\"title-heading-center\" style=\"margin:0;\">We use Next-Generation-Sequencing technologies to analyze genomes, exomes and panels.<\/h4><\/div><div class=\"fusion-text fusion-text-1\" style=\"text-align:center;\"><p>DNA sequencing includes the analysis of the genome, exome and gene panels customized to the customer&#8217;s needs. Our consolidated experience with a wide range of library preparation solutions allows us to provide consistent sequencing services. We are holders of a patent that allows you to select library preparation protocols, according to the best analytical coverage of the genes identified by customers.<\/p>\n<\/div><\/div><style type=\"text\/css\">.fusion-body .fusion-builder-column-1{width:100% !important;margin-top : 0px;margin-bottom : 30px;}.fusion-builder-column-1 > .fusion-column-wrapper {padding-top : 0px !important;padding-right : 0px !important;margin-right : 1.92%;padding-bottom : 0px !important;padding-left : 0px !important;margin-left : 1.92%;}@media only screen and (max-width:1024px) {.fusion-body .fusion-builder-column-1{width:100% !important;order : 0;}.fusion-builder-column-1 > .fusion-column-wrapper {margin-right : 1.92%;margin-left : 1.92%;}}@media only screen and (max-width:640px) {.fusion-body .fusion-builder-column-1{width:100% !important;order : 0;}.fusion-builder-column-1 > .fusion-column-wrapper {margin-right : 1.92%;margin-left : 1.92%;}}<\/style><\/div><\/div><style type=\"text\/css\">.fusion-body .fusion-flex-container.fusion-builder-row-2{ padding-top : 60px;margin-top : 0px;padding-right : 0px;padding-bottom : 25px;margin-bottom : 0px;padding-left : 0px;}<\/style><\/div><div class=\"fusion-fullwidth fullwidth-box fusion-builder-row-3 fusion-flex-container nonhundred-percent-fullwidth non-hundred-percent-height-scrolling\" style=\"background-color: #f0f0f0;background-position: center center;background-repeat: no-repeat;border-width: 0px 0px 0px 0px;border-color:#eae9e9;border-style:solid;\" ><div class=\"fusion-builder-row fusion-row fusion-flex-align-items-flex-start\" style=\"max-width:1352px;margin-left: calc(-4% \/ 2 );margin-right: calc(-4% \/ 2 );\"><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-2 fusion_builder_column_1_1 1_1 fusion-flex-column\"><div class=\"fusion-column-wrapper fusion-flex-justify-content-flex-start fusion-content-layout-column\" style=\"background-position:left top;background-repeat:no-repeat;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;padding: 0px 0px 0px 0px;\"><style type=\"text\/css\">@media only screen and (max-width:1024px) {.fusion-title.fusion-title-3{margin-top:0px!important; margin-right:0px!important;margin-bottom:15px!important;margin-left:0px!important;}}@media only screen and (max-width:640px) {.fusion-title.fusion-title-3{margin-top:0px!important; margin-right:0px!important;margin-bottom:20px!important; margin-left:0px!important;}}<\/style><div class=\"fusion-title title fusion-title-3 fusion-sep-none fusion-title-center fusion-title-text fusion-title-size-three\" style=\"margin-top:0px;margin-right:0px;margin-bottom:15px;margin-left:0px;\"><h3 class=\"title-heading-center\" style=\"margin:0;\">SAMPLE PREPARATION<\/h3><\/div><\/div><style type=\"text\/css\">.fusion-body .fusion-builder-column-2{width:100% !important;margin-top : 0px;margin-bottom : 30px;}.fusion-builder-column-2 > .fusion-column-wrapper {padding-top : 0px !important;padding-right : 0px !important;margin-right : 1.92%;padding-bottom : 0px !important;padding-left : 0px !important;margin-left : 1.92%;}@media only screen and (max-width:1024px) {.fusion-body .fusion-builder-column-2{width:100% !important;order : 0;}.fusion-builder-column-2 > .fusion-column-wrapper {margin-right : 1.92%;margin-left : 1.92%;}}@media only screen and (max-width:640px) {.fusion-body .fusion-builder-column-2{width:100% !important;order : 0;}.fusion-builder-column-2 > .fusion-column-wrapper {margin-right : 1.92%;margin-left : 1.92%;}}<\/style><\/div><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-3 fusion_builder_column_1_2 1_2 fusion-flex-column\"><div class=\"fusion-column-wrapper fusion-flex-justify-content-flex-start fusion-content-layout-column\" style=\"background-position:left top;background-repeat:no-repeat;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;padding: 0px 0px 0px 0px;\"><div class=\"fusion-text fusion-text-2\"><p><strong>EXTRACTION<\/strong><br \/>\nNucleic acid extraction is one of the critical steps to obtain consistent and high quality NGS data. We are able to select the best kits for extraction from the different matrices (eg blood, plasma, paraffin, fresh tissue and saliva).<\/p>\n<\/div><\/div><style type=\"text\/css\">.fusion-body .fusion-builder-column-3{width:50% !important;margin-top : 0px;margin-bottom : 0px;}.fusion-builder-column-3 > .fusion-column-wrapper {padding-top : 0px !important;padding-right : 0px !important;margin-right : 3.84%;padding-bottom : 0px !important;padding-left : 0px !important;margin-left : 3.84%;}@media only screen and (max-width:1024px) {.fusion-body .fusion-builder-column-3{width:100% !important;order : 0;}.fusion-builder-column-3 > .fusion-column-wrapper {margin-right : 1.92%;margin-left : 1.92%;}}@media only screen and (max-width:640px) {.fusion-body .fusion-builder-column-3{width:100% !important;order : 0;}.fusion-builder-column-3 > .fusion-column-wrapper {margin-right : 1.92%;margin-left : 1.92%;}}<\/style><\/div><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-4 fusion_builder_column_1_2 1_2 fusion-flex-column\"><div class=\"fusion-column-wrapper fusion-flex-justify-content-flex-start fusion-content-layout-column\" style=\"background-position:left top;background-repeat:no-repeat;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;padding: 0px 0px 0px 0px;\"><div class=\"fusion-text fusion-text-3\"><p><strong>QUALITY CHECK<\/strong><br \/>\nIn the sequencing process we apply stringent quality control protocols that allow us to inform the customer in real time on the progress of the analysis. The main tools for checking the quality and quantity of the extracted DNA we use are: NanoDrop, LabChip and Qubit. For paraffinized samples, we are also able to evaluate the amplificability and the degree of fragmentation of the DNA.<\/p>\n<\/div><\/div><style type=\"text\/css\">.fusion-body .fusion-builder-column-4{width:50% !important;margin-top : 0px;margin-bottom : 0px;}.fusion-builder-column-4 > .fusion-column-wrapper {padding-top : 0px !important;padding-right : 0px !important;margin-right : 3.84%;padding-bottom : 0px !important;padding-left : 0px !important;margin-left : 3.84%;}@media only screen and (max-width:1024px) {.fusion-body .fusion-builder-column-4{width:100% !important;order : 0;}.fusion-builder-column-4 > .fusion-column-wrapper {margin-right : 1.92%;margin-left : 1.92%;}}@media only screen and (max-width:640px) {.fusion-body .fusion-builder-column-4{width:100% !important;order : 0;}.fusion-builder-column-4 > .fusion-column-wrapper {margin-right : 1.92%;margin-left : 1.92%;}}<\/style><\/div><\/div><style type=\"text\/css\">.fusion-body .fusion-flex-container.fusion-builder-row-3{ padding-top : 60px;margin-top : 0px;padding-right : 0px;padding-bottom : 60px;margin-bottom : 0px;padding-left : 0px;}<\/style><\/div><div class=\"fusion-fullwidth fullwidth-box fusion-builder-row-4 fusion-flex-container fusion-parallax-none nonhundred-percent-fullwidth non-hundred-percent-height-scrolling\" style=\"background-color: rgba(255,255,255,0);background-image: url(&quot;https:\/\/www.personalgenomics.it\/en\/wp-content\/uploads\/sites\/2\/2021\/04\/banner-pc-laterale.jpg&quot;);background-position: center center;background-repeat: no-repeat;border-width: 0px 0px 0px 0px;border-color:#004b87;border-style:solid;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;\" ><div class=\"fusion-builder-row fusion-row fusion-flex-align-items-stretch\" style=\"max-width:1352px;margin-left: calc(-4% \/ 2 );margin-right: calc(-4% \/ 2 );\"><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-5 fusion_builder_column_1_2 1_2 fusion-flex-column fusion-no-large-visibility\"><div class=\"fusion-column-wrapper fusion-flex-justify-content-flex-start fusion-content-layout-column\" style=\"background-position:left top;background-repeat:no-repeat;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;background-color:rgba(255,255,255,0.8);padding: 30px 30px 30px 30px;\"><style type=\"text\/css\">@media only screen and (max-width:1024px) {.fusion-title.fusion-title-4{margin-top:0px!important; margin-right:0px!important;margin-bottom:15px!important;margin-left:0px!important;}}@media only screen and (max-width:640px) {.fusion-title.fusion-title-4{margin-top:0px!important; margin-right:0px!important;margin-bottom:20px!important; margin-left:0px!important;}}<\/style><div class=\"fusion-title title fusion-title-4 fusion-sep-none fusion-title-text fusion-title-size-three\" style=\"margin-top:0px;margin-right:0px;margin-bottom:15px;margin-left:0px;\"><h3 class=\"title-heading-left\" style=\"margin:0;\">SEQUENCING<\/h3><\/div><div class=\"fusion-text fusion-text-4 fusion-text-no-margin\" style=\"margin-bottom:30px;\"><p>The type of sequencing depends on the length of the library and the choice of capture kit. We mainly use Illumina sequencers, but we also have sequencing experience on other platforms, which can be used upon specific customer request.<\/p>\n<\/div><div class=\"fusion-text fusion-text-5 fusion-text-no-margin\" style=\"margin-bottom:30px;\"><p><strong>EXOME (WES) AND PANELS<br \/>\nSelection of capture kits<\/strong><br \/>\nWe constantly evaluate the performance of new enrichment platforms. This allows us to have a proprietary database, which can be queried to evaluate which kit is most suitable for each region of the exome, from which we obtained the system for selecting the capture kit and fragment length present in the patent.<\/p>\n<\/div><div class=\"fusion-text fusion-text-6 fusion-text-no-margin\" style=\"margin-bottom:30px;\"><p><strong>FULL GENOME (WGS)<\/strong><br \/>\nWe use PCR-free libraries to ensure the maximum possible coverage. Genomic libraries produced with protocols that involve the use of PCR, in fact, show a more irregular or absent coverage, which for us at Personal Genomics is not enough.<\/p>\n<\/div><\/div><style type=\"text\/css\">.fusion-body .fusion-builder-column-5{width:50% !important;margin-top : 0px;margin-bottom : 0px;}.fusion-builder-column-5 > .fusion-column-wrapper {padding-top : 30px !important;padding-right : 30px !important;margin-right : 3.84%;padding-bottom : 30px !important;padding-left : 30px !important;margin-left : 3.84%;}@media only screen and (max-width:1024px) {.fusion-body .fusion-builder-column-5{width:100% !important;order : 0;}.fusion-builder-column-5 > .fusion-column-wrapper {margin-right : 1.92%;margin-left : 1.92%;}}@media only screen and (max-width:640px) {.fusion-body .fusion-builder-column-5{width:100% !important;order : 0;}.fusion-builder-column-5 > .fusion-column-wrapper {margin-right : 1.92%;margin-left : 1.92%;}}<\/style><\/div><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-6 fusion_builder_column_1_2 1_2 fusion-flex-column fusion-no-small-visibility fusion-no-medium-visibility\"><div class=\"fusion-column-wrapper fusion-flex-justify-content-flex-start fusion-content-layout-column\" style=\"background-position:left top;background-repeat:no-repeat;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;padding: 0px 0px 0px 0px;\"><style type=\"text\/css\">@media only screen and (max-width:1024px) {.fusion-title.fusion-title-5{margin-top:0px!important; margin-right:0px!important;margin-bottom:15px!important;margin-left:0px!important;}}@media only screen and (max-width:640px) {.fusion-title.fusion-title-5{margin-top:0px!important; margin-right:0px!important;margin-bottom:20px!important; margin-left:0px!important;}}<\/style><div class=\"fusion-title title fusion-title-5 fusion-sep-none fusion-title-text fusion-title-size-three\" style=\"margin-top:0px;margin-right:0px;margin-bottom:15px;margin-left:0px;\"><h3 class=\"title-heading-left\" style=\"margin:0;\">SEQUENCING<\/h3><\/div><div class=\"fusion-text fusion-text-7 fusion-text-no-margin\" style=\"margin-bottom:30px;\"><p>The type of sequencing depends on the length of the library and the choice of capture kit. We mainly use Illumina sequencers, but we also have sequencing experience on other platforms, which can be used upon specific customer request.<\/p>\n<\/div><div class=\"fusion-text fusion-text-8 fusion-text-no-margin\" style=\"margin-bottom:30px;\"><p><strong>EXOME (WES) AND PANELS<br \/>\nSelection of capture kits<\/strong><br \/>\nWe constantly evaluate the performance of new enrichment platforms. This allows us to have a proprietary database, which can be queried to evaluate which kit is most suitable for each region of the exome, from which we obtained the system for selecting the capture kit and fragment length present in the patent.<\/p>\n<\/div><div class=\"fusion-text fusion-text-9 fusion-text-no-margin\" style=\"margin-bottom:30px;\"><p><strong>FULL GENOME (WGS)<\/strong><br \/>\nWe use PCR-free libraries to ensure the maximum possible coverage. Genomic libraries produced with protocols that involve the use of PCR, in fact, show a more irregular or absent coverage, which for us at Personal Genomics is not enough.<\/p>\n<\/div><\/div><style type=\"text\/css\">.fusion-body .fusion-builder-column-6{width:50% !important;margin-top : 0px;margin-bottom : 0px;}.fusion-builder-column-6 > .fusion-column-wrapper {padding-top : 0px !important;padding-right : 0px !important;margin-right : 3.84%;padding-bottom : 0px !important;padding-left : 0px !important;margin-left : 3.84%;}@media only screen and (max-width:1024px) {.fusion-body .fusion-builder-column-6{width:100% !important;order : 0;}.fusion-builder-column-6 > .fusion-column-wrapper {margin-right : 1.92%;margin-left : 1.92%;}}@media only screen and (max-width:640px) {.fusion-body .fusion-builder-column-6{width:100% !important;order : 0;}.fusion-builder-column-6 > .fusion-column-wrapper {margin-right : 1.92%;margin-left : 1.92%;}}<\/style><\/div><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-7 fusion_builder_column_1_2 1_2 fusion-flex-column\"><div class=\"fusion-column-wrapper fusion-flex-justify-content-flex-start fusion-content-layout-column\" style=\"background-position:left top;background-repeat:no-repeat;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;padding: 0px 0px 0px 0px;\"><\/div><style type=\"text\/css\">.fusion-body .fusion-builder-column-7{width:50% !important;margin-top : 0px;margin-bottom : 0px;}.fusion-builder-column-7 > .fusion-column-wrapper {padding-top : 0px !important;padding-right : 0px !important;margin-right : 3.84%;padding-bottom : 0px !important;padding-left : 0px !important;margin-left : 3.84%;}@media only screen and (max-width:1024px) {.fusion-body .fusion-builder-column-7{width:100% !important;order : 0;}.fusion-builder-column-7 > .fusion-column-wrapper {margin-right : 1.92%;margin-left : 1.92%;}}@media only screen and (max-width:640px) {.fusion-body .fusion-builder-column-7{width:100% !important;order : 0;}.fusion-builder-column-7 > .fusion-column-wrapper {margin-right : 1.92%;margin-left : 1.92%;}}<\/style><\/div><\/div><style type=\"text\/css\">.fusion-body .fusion-flex-container.fusion-builder-row-4{ padding-top : 80px;margin-top : 0px;padding-right : 0px;padding-bottom : 80px;margin-bottom : 0px;padding-left : 0px;}<\/style><\/div><div class=\"fusion-fullwidth fullwidth-box fusion-builder-row-5 fusion-flex-container nonhundred-percent-fullwidth non-hundred-percent-height-scrolling\" style=\"background-color: rgba(255,255,255,0);background-position: center center;background-repeat: no-repeat;border-width: 0px 0px 0px 0px;border-color:#eae9e9;border-style:solid;\" ><div class=\"fusion-builder-row fusion-row fusion-flex-align-items-flex-start\" style=\"max-width:1352px;margin-left: calc(-4% \/ 2 );margin-right: calc(-4% \/ 2 );\"><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-8 fusion_builder_column_1_2 1_2 fusion-flex-column\"><div class=\"fusion-column-wrapper fusion-flex-justify-content-flex-start fusion-content-layout-column\" style=\"background-position:left top;background-repeat:no-repeat;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;padding: 0px 0px 0px 0px;\"><div class=\"fusion-text fusion-text-10 fusion-text-no-margin\" style=\"margin-bottom:30px;\"><p><strong>IMPROVE GENOTYPIZZABILITY<\/strong><br \/>\nTo achieve maximum genotypability, we have developed capture library preparation protocols capable of modifying fragment length. By lengthening the fragment to an average length of about 300-350bp (against the 150-200bp foreseen by the standard protocols), it is possible to sequence with the 150PE protocol and, consequently, to improve the mappability of the sequences and the subsequent calling of the variants.<\/p>\n<\/div><\/div><style type=\"text\/css\">.fusion-body .fusion-builder-column-8{width:50% !important;margin-top : 0px;margin-bottom : 0px;}.fusion-builder-column-8 > .fusion-column-wrapper {padding-top : 0px !important;padding-right : 0px !important;margin-right : 3.84%;padding-bottom : 0px !important;padding-left : 0px !important;margin-left : 3.84%;}@media only screen and (max-width:1024px) {.fusion-body .fusion-builder-column-8{width:100% !important;order : 0;}.fusion-builder-column-8 > .fusion-column-wrapper {margin-right : 1.92%;margin-left : 1.92%;}}@media only screen and (max-width:640px) {.fusion-body .fusion-builder-column-8{width:100% !important;order : 0;}.fusion-builder-column-8 > .fusion-column-wrapper {margin-right : 1.92%;margin-left : 1.92%;}}<\/style><\/div><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-9 fusion_builder_column_1_2 1_2 fusion-flex-column\"><div class=\"fusion-column-wrapper fusion-flex-justify-content-flex-start fusion-content-layout-column\" style=\"background-position:left top;background-repeat:no-repeat;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;padding: 0px 0px 0px 0px;\"><div class=\"fusion-text fusion-text-11 fusion-text-no-margin\" style=\"margin-bottom:30px;\"><p><strong>PANEL DESIGN<\/strong><br \/>\nThe coverage and genotyping information of the different capture kits as the average fragment length varies have allowed the development of an application, which allows you to choose the best possible configuration in the sequencing of a gene pool.<br \/>\n<strong>We at Personal Genomics have patented our method of producing libraries.<\/strong><\/p>\n<\/div><\/div><style type=\"text\/css\">.fusion-body .fusion-builder-column-9{width:50% !important;margin-top : 0px;margin-bottom : 0px;}.fusion-builder-column-9 > .fusion-column-wrapper {padding-top : 0px !important;padding-right : 0px !important;margin-right : 3.84%;padding-bottom : 0px !important;padding-left : 0px !important;margin-left : 3.84%;}@media only screen and (max-width:1024px) {.fusion-body .fusion-builder-column-9{width:100% !important;order : 0;}.fusion-builder-column-9 > .fusion-column-wrapper {margin-right : 1.92%;margin-left : 1.92%;}}@media only screen and (max-width:640px) {.fusion-body .fusion-builder-column-9{width:100% !important;order : 0;}.fusion-builder-column-9 > .fusion-column-wrapper {margin-right : 1.92%;margin-left : 1.92%;}}<\/style><\/div><\/div><style type=\"text\/css\">.fusion-body .fusion-flex-container.fusion-builder-row-5{ padding-top : 60px;margin-top : 0px;padding-right : 0px;padding-bottom : 60px;margin-bottom : 0px;padding-left : 0px;}<\/style><\/div><div class=\"fusion-fullwidth fullwidth-box fusion-builder-row-6 fusion-flex-container nonhundred-percent-fullwidth non-hundred-percent-height-scrolling\" style=\"background-color: #f0f0f0;background-position: center center;background-repeat: no-repeat;border-width: 0px 0px 0px 0px;border-color:#eae9e9;border-style:solid;\" ><div class=\"fusion-builder-row fusion-row fusion-flex-align-items-flex-start\" style=\"max-width:1352px;margin-left: calc(-4% \/ 2 );margin-right: calc(-4% \/ 2 );\"><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-10 fusion_builder_column_1_1 1_1 fusion-flex-column\"><div class=\"fusion-column-wrapper fusion-flex-justify-content-flex-start fusion-content-layout-column\" style=\"background-position:left top;background-repeat:no-repeat;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;padding: 0px 0px 0px 0px;\"><style type=\"text\/css\">@media only screen and (max-width:1024px) {.fusion-title.fusion-title-6{margin-top:0px!important; margin-right:0px!important;margin-bottom:60px!important;margin-left:0px!important;}}@media only screen and (max-width:640px) {.fusion-title.fusion-title-6{margin-top:0px!important; margin-right:0px!important;margin-bottom:20px!important; margin-left:0px!important;}}<\/style><div class=\"fusion-title title fusion-title-6 fusion-sep-none fusion-title-center fusion-title-text fusion-title-size-three\" style=\"margin-top:0px;margin-right:0px;margin-bottom:60px;margin-left:0px;\"><h3 class=\"title-heading-center\" style=\"margin:0;\">BIOINFORMATIC ANALYSIS<\/h3><\/div>\n<div class=\"table-2\">\n<table width=\"100%\">\n<tbody>\n<tr>\n<td align=\"left\"><\/td>\n<td align=\"left\"><strong>I level bioinformatic analysis<\/strong><\/td>\n<td align=\"left\"><strong>II level bioinformatic analysis<\/strong><\/td>\n<td align=\"left\"><strong>III level bioinformatic analysis<\/strong><\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Demultiplexing<\/td>\n<td style=\"background-color: #70bc82;\" align=\"left\"><\/td>\n<td style=\"background-color: #70bc82;\" align=\"left\"><\/td>\n<td style=\"background-color: #70bc82;\" align=\"left\"><\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Production of FastQ files<\/td>\n<td style=\"background-color: #70bc82;\" align=\"left\"><\/td>\n<td style=\"background-color: #70bc82;\" align=\"left\"><\/td>\n<td style=\"background-color: #70bc82;\" align=\"left\"><\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Alignment<\/td>\n<td align=\"left\"><\/td>\n<td style=\"background-color: #70bc82;\" align=\"left\"><\/td>\n<td style=\"background-color: #70bc82;\" align=\"left\"><\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Call of variants<\/td>\n<td align=\"left\"><\/td>\n<td style=\"background-color: #70bc82;\" align=\"left\"><\/td>\n<td style=\"background-color: #70bc82;\" align=\"left\"><\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Annotation<\/td>\n<td align=\"left\"><\/td>\n<td align=\"left\"><\/td>\n<td style=\"background-color: #70bc82;\" align=\"left\"><\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Variant prioritization<\/td>\n<td align=\"left\"><\/td>\n<td align=\"left\"><\/td>\n<td style=\"background-color: #70bc82;\" align=\"left\"><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div><style type=\"text\/css\">.fusion-body .fusion-builder-column-10{width:100% !important;margin-top : 0px;margin-bottom : 60px;}.fusion-builder-column-10 > .fusion-column-wrapper {padding-top : 0px !important;padding-right : 0px !important;margin-right : 1.92%;padding-bottom : 0px !important;padding-left : 0px !important;margin-left : 1.92%;}@media only screen and (max-width:1024px) {.fusion-body .fusion-builder-column-10{width:100% !important;order : 0;}.fusion-builder-column-10 > .fusion-column-wrapper {margin-right : 1.92%;margin-left : 1.92%;}}@media only screen and (max-width:640px) {.fusion-body .fusion-builder-column-10{width:100% !important;order : 0;}.fusion-builder-column-10 > .fusion-column-wrapper {margin-right : 1.92%;margin-left : 1.92%;}}<\/style><\/div><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-11 fusion_builder_column_1_2 1_2 fusion-flex-column\"><div class=\"fusion-column-wrapper fusion-flex-justify-content-flex-start fusion-content-layout-column\" style=\"background-position:left top;background-repeat:no-repeat;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;padding: 0px 0px 0px 0px;\"><div class=\"fusion-text fusion-text-12 fusion-text-no-margin\" style=\"margin-bottom:30px;\"><p><strong>ALIGNMENT AND CALL OF VARIANTS<\/strong><\/p>\n<ul>\n<li>Alignment on reference (hg19 or hg38) and production of BAM files;<\/li>\n<li>Call variants with BWA-mem \/ GATK4 pipeline and production of VCF files + genotyped regions.<\/li>\n<\/ul>\n<p>The VCF reports information on the variants called in an individual and assumes that the lack of information is equivalent to the homozygous presence of the reference allele.<br \/>\nThis assumption is false because a position may not be present in the VCF also due to lack of adequate coverage in the panel or due to low mappability. For this we provide a file that reports the genotyped regions.<br \/>\nAs an alternative to the standard BWA-mem \/ GATK4 pipeline, a gVCF file can be produced using the iSAACalign \/ strelka pipeline.<\/p>\n<\/div><\/div><style type=\"text\/css\">.fusion-body .fusion-builder-column-11{width:50% !important;margin-top : 0px;margin-bottom : 0px;}.fusion-builder-column-11 > .fusion-column-wrapper {padding-top : 0px !important;padding-right : 0px !important;margin-right : 3.84%;padding-bottom : 0px !important;padding-left : 0px !important;margin-left : 3.84%;}@media only screen and (max-width:1024px) {.fusion-body .fusion-builder-column-11{width:100% !important;order : 0;}.fusion-builder-column-11 > .fusion-column-wrapper {margin-right : 1.92%;margin-left : 1.92%;}}@media only screen and (max-width:640px) {.fusion-body .fusion-builder-column-11{width:100% !important;order : 0;}.fusion-builder-column-11 > .fusion-column-wrapper {margin-right : 1.92%;margin-left : 1.92%;}}<\/style><\/div><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-12 fusion_builder_column_1_2 1_2 fusion-flex-column\"><div class=\"fusion-column-wrapper fusion-flex-justify-content-flex-start fusion-content-layout-column\" style=\"background-position:left top;background-repeat:no-repeat;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;padding: 0px 0px 0px 0px;\"><div class=\"fusion-text fusion-text-13 fusion-text-no-margin\" style=\"margin-bottom:30px;\"><p><strong>COVERAGE AND GENOTYPIZABILITY ANALYSIS<\/strong><br \/>\nWe do NOT consider the theoretical coverages, but only the real ones, thus excluding:<\/p>\n<ul>\n<li>Off target<\/li>\n<li>Padded Region<\/li>\n<li>Duplicates<\/li>\n<\/ul>\n<p>We at Personal Genomics have developed a pipeline to provide a detailed analysis of the coverage and genotyping for all coding regions in RefSeq in the case of a complete exome or the target region in the case of a panel.<\/p>\n<\/div><\/div><style type=\"text\/css\">.fusion-body .fusion-builder-column-12{width:50% !important;margin-top : 0px;margin-bottom : 0px;}.fusion-builder-column-12 > .fusion-column-wrapper {padding-top : 0px !important;padding-right : 0px !important;margin-right : 3.84%;padding-bottom : 0px !important;padding-left : 0px !important;margin-left : 3.84%;}@media only screen and (max-width:1024px) {.fusion-body .fusion-builder-column-12{width:100% !important;order : 0;}.fusion-builder-column-12 > .fusion-column-wrapper {margin-right : 1.92%;margin-left : 1.92%;}}@media only screen and (max-width:640px) {.fusion-body .fusion-builder-column-12{width:100% !important;order : 0;}.fusion-builder-column-12 > .fusion-column-wrapper {margin-right : 1.92%;margin-left : 1.92%;}}<\/style><\/div><\/div><style type=\"text\/css\">.fusion-body .fusion-flex-container.fusion-builder-row-6{ padding-top : 60px;margin-top : 0px;padding-right : 0px;padding-bottom : 60px;margin-bottom : 0px;padding-left : 0px;}<\/style><\/div><div class=\"fusion-fullwidth fullwidth-box fusion-builder-row-7 fusion-flex-container nonhundred-percent-fullwidth non-hundred-percent-height-scrolling\" style=\"background-color: rgba(255,255,255,0);background-position: center center;background-repeat: no-repeat;border-width: 0px 0px 0px 0px;border-color:#eae9e9;border-style:solid;\" ><div class=\"fusion-builder-row fusion-row fusion-flex-align-items-center\" style=\"max-width:1352px;margin-left: calc(-4% \/ 2 );margin-right: calc(-4% \/ 2 );\"><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-13 fusion_builder_column_1_2 1_2 fusion-flex-column\"><div class=\"fusion-column-wrapper fusion-flex-justify-content-flex-start fusion-content-layout-column\" style=\"background-position:left top;background-repeat:no-repeat;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;padding: 0px 0px 0px 0px;\"><div style=\"text-align:center;\"><span class=\" fusion-imageframe imageframe-none imageframe-1 hover-type-none\"><img width=\"500\" height=\"444\" title=\"brochure-servizi-ngs-custom\" src=\"https:\/\/www.personalgenomics.it\/en\/wp-content\/uploads\/sites\/2\/2021\/05\/brochure-servizi-ngs-custom.png\" alt class=\"img-responsive wp-image-1236\" srcset=\"https:\/\/www.personalgenomics.it\/en\/wp-content\/uploads\/sites\/2\/2021\/05\/brochure-servizi-ngs-custom-200x178.png 200w, https:\/\/www.personalgenomics.it\/en\/wp-content\/uploads\/sites\/2\/2021\/05\/brochure-servizi-ngs-custom-400x355.png 400w, https:\/\/www.personalgenomics.it\/en\/wp-content\/uploads\/sites\/2\/2021\/05\/brochure-servizi-ngs-custom.png 500w\" sizes=\"(max-width: 1024px) 100vw, (max-width: 640px) 100vw, 500px\" \/><\/span><\/div><\/div><style type=\"text\/css\">.fusion-body .fusion-builder-column-13{width:50% !important;margin-top : 0px;margin-bottom : 0px;}.fusion-builder-column-13 > .fusion-column-wrapper {padding-top : 0px !important;padding-right : 0px !important;margin-right : 3.84%;padding-bottom : 0px !important;padding-left : 0px !important;margin-left : 3.84%;}@media only screen and (max-width:1024px) {.fusion-body .fusion-builder-column-13{width:100% !important;order : 0;}.fusion-builder-column-13 > .fusion-column-wrapper {margin-right : 1.92%;margin-left : 1.92%;}}@media only screen and (max-width:640px) {.fusion-body .fusion-builder-column-13{width:100% !important;order : 0;}.fusion-builder-column-13 > .fusion-column-wrapper {margin-right : 1.92%;margin-left : 1.92%;}}<\/style><\/div><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-14 fusion_builder_column_1_2 1_2 fusion-flex-column\"><div class=\"fusion-column-wrapper fusion-flex-justify-content-flex-start fusion-content-layout-column\" style=\"background-position:left top;background-repeat:no-repeat;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;padding: 0px 0px 0px 0px;\"><style type=\"text\/css\">@media only screen and (max-width:1024px) {.fusion-title.fusion-title-7{margin-top:0px!important; margin-right:0px!important;margin-bottom:15px!important;margin-left:0px!important;}}@media only screen and (max-width:640px) {.fusion-title.fusion-title-7{margin-top:0px!important; margin-right:0px!important;margin-bottom:20px!important; margin-left:0px!important;}}<\/style><div class=\"fusion-title title fusion-title-7 fusion-sep-none fusion-title-center fusion-title-text fusion-title-size-four\" style=\"margin-top:0px;margin-right:0px;margin-bottom:15px;margin-left:0px;\"><h4 class=\"title-heading-center\" style=\"margin:0;\">Do you want to know more details about our DNA sequencing service?<\/h4><\/div><div style=\"text-align:center;\"><style>.fusion-body .fusion-button.button-1{border-radius:8px 8px 8px 8px;}<\/style><a class=\"fusion-button button-flat fusion-button-default-size button-default button-1 fusion-button-default-span fusion-button-default-type\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.personalgenomics.it\/pdf\/personal-genomics-brochure-servizi-NGS-EN.pdf\"><span class=\"fusion-button-text\">download the leaflet<\/span><\/a><\/div><\/div><style type=\"text\/css\">.fusion-body .fusion-builder-column-14{width:50% !important;margin-top : 0px;margin-bottom : 0px;}.fusion-builder-column-14 > .fusion-column-wrapper {padding-top : 0px !important;padding-right : 0px !important;margin-right : 3.84%;padding-bottom : 0px !important;padding-left : 0px !important;margin-left : 3.84%;}@media only screen and (max-width:1024px) {.fusion-body .fusion-builder-column-14{width:100% !important;order : 0;}.fusion-builder-column-14 > .fusion-column-wrapper {margin-right : 1.92%;margin-left : 1.92%;}}@media only screen and (max-width:640px) {.fusion-body .fusion-builder-column-14{width:100% !important;order : 0;}.fusion-builder-column-14 > .fusion-column-wrapper {margin-right : 1.92%;margin-left : 1.92%;}}<\/style><\/div><\/div><style type=\"text\/css\">.fusion-body .fusion-flex-container.fusion-builder-row-7{ padding-top : 0px;margin-top : 0px;padding-right : 0px;padding-bottom : 0px;margin-bottom : 0px;padding-left : 0px;}<\/style><\/div><div class=\"fusion-fullwidth fullwidth-box fusion-builder-row-8 fusion-flex-container nonhundred-percent-fullwidth non-hundred-percent-height-scrolling\" style=\"background-color: #f0f0f0;background-position: center center;background-repeat: no-repeat;border-width: 0px 0px 0px 0px;border-color:#004b87;border-style:solid;\" ><div class=\"fusion-builder-row fusion-row fusion-flex-align-items-stretch\" style=\"max-width:1352px;margin-left: calc(-4% \/ 2 );margin-right: calc(-4% \/ 2 );\"><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-15 fusion_builder_column_1_5 1_5 fusion-flex-column\"><div class=\"fusion-column-wrapper fusion-flex-justify-content-flex-start fusion-content-layout-column\" style=\"background-position:left top;background-repeat:no-repeat;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;padding: 0px 0px 0px 0px;\"><\/div><style type=\"text\/css\">.fusion-body .fusion-builder-column-15{width:20% !important;margin-top : 0px;margin-bottom : 0px;}.fusion-builder-column-15 > .fusion-column-wrapper {padding-top : 0px !important;padding-right : 0px !important;margin-right : 9.6%;padding-bottom : 0px !important;padding-left : 0px !important;margin-left : 9.6%;}@media only screen and (max-width:1024px) {.fusion-body .fusion-builder-column-15{width:100% !important;order : 0;}.fusion-builder-column-15 > .fusion-column-wrapper {margin-right : 1.92%;margin-left : 1.92%;}}@media only screen and (max-width:640px) {.fusion-body .fusion-builder-column-15{width:100% !important;order : 0;}.fusion-builder-column-15 > .fusion-column-wrapper {margin-right : 1.92%;margin-left : 1.92%;}}<\/style><\/div><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-16 fusion_builder_column_3_5 3_5 fusion-flex-column fusion-column-no-min-height\"><div class=\"fusion-column-wrapper fusion-flex-justify-content-flex-start fusion-content-layout-column\" style=\"background-position:left top;background-repeat:no-repeat;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;padding: 0px 0px 0px 0px;\"><style type=\"text\/css\">@media only screen and (max-width:1024px) {.fusion-title.fusion-title-8{margin-top:0px!important; margin-right:0px!important;margin-bottom:15px!important;margin-left:0px!important;}}@media only screen and (max-width:640px) {.fusion-title.fusion-title-8{margin-top:0px!important; margin-right:0px!important;margin-bottom:20px!important; margin-left:0px!important;}}<\/style><div class=\"fusion-title title fusion-title-8 fusion-sep-none fusion-title-center fusion-title-text fusion-title-size-three\" style=\"margin-top:0px;margin-right:0px;margin-bottom:15px;margin-left:0px;\"><h3 class=\"title-heading-center\" style=\"margin:0;\">Contact us for addittional information<\/h3><\/div><div role=\"form\" class=\"wpcf7\" id=\"wpcf7-f1223-o1\" lang=\"it-IT\" dir=\"ltr\">\n<div class=\"screen-reader-response\"><p role=\"status\" aria-live=\"polite\" aria-atomic=\"true\"><\/p> <ul><\/ul><\/div>\n<form action=\"\/en\/wp-json\/wp\/v2\/pages\/1110#wpcf7-f1223-o1\" method=\"post\" class=\"wpcf7-form init\" enctype=\"multipart\/form-data\" novalidate=\"novalidate\" data-status=\"init\">\n<div style=\"display: none;\">\n<input type=\"hidden\" name=\"_wpcf7\" value=\"1223\" \/>\n<input type=\"hidden\" name=\"_wpcf7_version\" value=\"5.6.1\" \/>\n<input type=\"hidden\" name=\"_wpcf7_locale\" value=\"it_IT\" \/>\n<input type=\"hidden\" name=\"_wpcf7_unit_tag\" value=\"wpcf7-f1223-o1\" \/>\n<input type=\"hidden\" name=\"_wpcf7_container_post\" value=\"0\" \/>\n<input type=\"hidden\" name=\"_wpcf7_posted_data_hash\" value=\"\" \/>\n<input type=\"hidden\" name=\"_wpcf7cf_hidden_group_fields\" value=\"[]\" \/>\n<input type=\"hidden\" name=\"_wpcf7cf_hidden_groups\" value=\"[]\" \/>\n<input type=\"hidden\" name=\"_wpcf7cf_visible_groups\" value=\"[]\" \/>\n<input type=\"hidden\" name=\"_wpcf7cf_repeaters\" value=\"[]\" \/>\n<input type=\"hidden\" name=\"_wpcf7cf_steps\" value=\"{}\" \/>\n<input type=\"hidden\" name=\"_wpcf7cf_options\" value=\"{&quot;form_id&quot;:1223,&quot;conditions&quot;:[{&quot;then_field&quot;:&quot;gruppo-quanti-geni&quot;,&quot;and_rules&quot;:[{&quot;if_field&quot;:&quot;tipo-analisi&quot;,&quot;operator&quot;:&quot;equals&quot;,&quot;if_value&quot;:&quot;Paneles of custom genes&quot;}]}],&quot;settings&quot;:{&quot;animation&quot;:&quot;yes&quot;,&quot;animation_intime&quot;:200,&quot;animation_outtime&quot;:200,&quot;conditions_ui&quot;:&quot;normal&quot;,&quot;notice_dismissed&quot;:false}}\" \/>\n<input type=\"hidden\" name=\"_wpcf7_recaptcha_response\" value=\"\" \/>\n<\/div>\n<style>\n                .uacf7-uacf7style-1223 label {\n                    color: ;\n                    background-color: ;\n                    font-size: px;\n                    font-family: ;\n                    font-style: normal;\n                    font-weight: normal;\n                    padding-top: px;\n                    padding-right: px;\n                    padding-bottom: px;\n                    padding-left: px;\n                    margin-top: px;\n                    margin-right: px;\n                    margin-bottom: px;\n                    margin-left: px;\n                }\n                .uacf7-uacf7style-1223 input[type=\"email\"],\n                .uacf7-uacf7style-1223 input[type=\"number\"],\n                .uacf7-uacf7style-1223 input[type=\"password\"],\n                .uacf7-uacf7style-1223 input[type=\"search\"],\n                .uacf7-uacf7style-1223 input[type=\"tel\"],\n                .uacf7-uacf7style-1223 input[type=\"text\"],\n                .uacf7-uacf7style-1223 input[type=\"url\"],\n                .uacf7-uacf7style-1223 input[type=\"date\"],\n                .uacf7-uacf7style-1223 select,\n                .uacf7-uacf7style-1223 textarea {\n                    color: ;\n                    background-color: ;\n                    font-size: px;\n                    font-family: ;\n                    font-style: normal;\n                    font-weight: normal;\n                    height: px;\n                    border-width: px;\n                    border-color: ;\n                    border-style: nessuno;\n                    border-radius: px;\n                    padding-top: px;\n                    padding-right: px;\n                    padding-bottom: px;\n                    padding-left: px;\n                    margin-top: px;\n                    margin-right: px;\n                    margin-bottom: px;\n                    margin-left: px;\n                }\n                .uacf7-uacf7style-1223 .wpcf7-radio span,\n                .uacf7-uacf7style-1223 .wpcf7-checkbox span {\n                    color: ;\n                    font-size: px;\n                    font-family: ;\n                    font-style: normal;\n                    font-weight: normal;\n                }\n                .uacf7-uacf7style-1223 textarea {\n                    height: px;\n                }\n                .wpcf7-form-control-wrap select {\n                    width: 100%;\n                }\n                .uacf7-uacf7style-1223 input[type=\"submit\"] {\n                    color: ;\n                    background-color: ;\n                    font-size: px;\n                    font-family: ;\n                    font-style: normal;\n                    font-weight: normal;\n                    border-width: px;\n                    border-color: ;\n                    border-style: nessuno;\n                    border-radius: px;\n                    width: ;\n                    padding-top: px;\n                    padding-right: px;\n                    padding-bottom: px;\n                    padding-left: px;\n                    margin-top: px;\n                    margin-right: px;\n                    margin-bottom: px;\n                    margin-left: px;\n                }\n                .uacf7-uacf7style-1223 input[type=\"submit\"]:hover {\n                    color: ;\n                    background-color: ;\n                    border-color: ;\n                }\n                            <\/style>\n<div class=\"uacf7-uacf7style-1223\">\n<div class=\"uacf7-form-1223\">\n<div class=\"col-sm-6\" style=\"margin-top: 15px;\"><label> <span class=\"wpcf7-form-control-wrap\" data-name=\"your-name\"><input type=\"text\" name=\"your-name\" value=\"\" size=\"40\" class=\"wpcf7-form-control wpcf7-text wpcf7-validates-as-required\" aria-required=\"true\" aria-invalid=\"false\" placeholder=\"First name *\" \/><\/span> <\/label><\/div>\n<div class=\"col-sm-6\" style=\"margin-top: 15px;\"><label> <span class=\"wpcf7-form-control-wrap\" data-name=\"cognome\"><input type=\"text\" name=\"cognome\" value=\"\" size=\"40\" class=\"wpcf7-form-control wpcf7-text wpcf7-validates-as-required\" aria-required=\"true\" aria-invalid=\"false\" placeholder=\"Last name *\" \/><\/span> <\/label><\/div>\n<div class=\"col-sm-12\" style=\"margin-top: 15px;\"><label> <span class=\"wpcf7-form-control-wrap\" data-name=\"your-email\"><input type=\"email\" name=\"your-email\" value=\"\" size=\"40\" class=\"wpcf7-form-control wpcf7-text wpcf7-email wpcf7-validates-as-required wpcf7-validates-as-email\" aria-required=\"true\" aria-invalid=\"false\" placeholder=\"Email *\" \/><\/span> <\/label><\/div>\n<div class=\"col-sm-6\" style=\"margin-top: 15px;\"><label> <span class=\"wpcf7-form-control-wrap\" data-name=\"telefono\"><input type=\"tel\" name=\"telefono\" value=\"\" size=\"40\" class=\"wpcf7-form-control wpcf7-text wpcf7-tel wpcf7-validates-as-required wpcf7-validates-as-tel\" aria-required=\"true\" aria-invalid=\"false\" placeholder=\"Phone *\" \/><\/span> <\/label><\/div>\n<div class=\"col-sm-6\" style=\"margin-top: 15px;\"><label> <span class=\"wpcf7-form-control-wrap\" data-name=\"regione\"><input type=\"text\" name=\"regione\" value=\"\" size=\"40\" class=\"wpcf7-form-control wpcf7-text wpcf7-validates-as-required\" aria-required=\"true\" aria-invalid=\"false\" placeholder=\"Country *\" \/><\/span> <\/label><\/div>\n<div class=\"col-sm-6\" style=\"margin-top:15px;\"><span class=\"wpcf7-form-control-wrap\" data-name=\"tipo-analisi\"><select name=\"tipo-analisi\" class=\"wpcf7-form-control wpcf7-select\" aria-invalid=\"false\"><option value=\"\">Kind of required analysis<\/option><option value=\"WES\">WES<\/option><option value=\"WGS\">WGS<\/option><option value=\"Paneles of custom genes\">Paneles of custom genes<\/option><\/select><\/span><\/div>\n<div data-id=\"gruppo-quanti-geni\" data-orig_data_id=\"gruppo-quanti-geni\"  data-class=\"wpcf7cf_group\">\n<div class=\"col-sm-6\" style=\"margin-top:15px;\"><label> <span class=\"wpcf7-form-control-wrap\" data-name=\"numero-geni\"><input type=\"number\" name=\"numero-geni\" value=\"\" class=\"wpcf7-form-control wpcf7-number wpcf7-validates-as-number\" aria-invalid=\"false\" placeholder=\"Gene number\" \/><\/span> <\/label><\/div>\n<\/div>\n<div class=\"col-sm-6\" style=\"margin-top:15px;\"><label> <span class=\"wpcf7-form-control-wrap\" data-name=\"numero-campioni\"><input type=\"number\" name=\"numero-campioni\" value=\"\" class=\"wpcf7-form-control wpcf7-number wpcf7-validates-as-number\" aria-invalid=\"false\" placeholder=\"Sample number\" \/><\/span><\/label><\/div>\n<div class=\"col-sm-6\" style=\"margin-top:15px;\"><label> <span class=\"wpcf7-form-control-wrap\" data-name=\"dati-sequenziamento\"><input type=\"number\" name=\"dati-sequenziamento\" value=\"\" class=\"wpcf7-form-control wpcf7-number wpcf7-validates-as-number\" aria-invalid=\"false\" placeholder=\"Sequencing data\" \/><\/span><\/label><\/div>\n<div class=\"col-sm-12\" style=\"margin-top: 15px;\"><label> <span class=\"wpcf7-form-control-wrap\" data-name=\"your-message\"><textarea name=\"your-message\" cols=\"40\" rows=\"10\" class=\"wpcf7-form-control wpcf7-textarea\" aria-invalid=\"false\" placeholder=\"Message\"><\/textarea><\/span> <\/label><\/div>\n<div class=\"col-sm-12\" style=\"margin-top:10px;\"><label> Attach document <span class=\"wpcf7-form-control-wrap\" data-name=\"documento\"><input type=\"file\" name=\"documento\" size=\"40\" class=\"wpcf7-form-control wpcf7-file\" accept=\".pdf,.excel\" aria-invalid=\"false\" \/><\/span><\/label><\/div>\n<div class=\"col-sm-12\" style=\"font-size:13px; line-height:30px; margin-top: 10px; margin-bottom: 10px;\"><span class=\"wpcf7-form-control-wrap\" data-name=\"newsletter\"><span class=\"wpcf7-form-control wpcf7-acceptance optional\"><span class=\"wpcf7-list-item\"><label><input type=\"checkbox\" name=\"newsletter\" value=\"1\" aria-invalid=\"false\" \/><span class=\"wpcf7-list-item-label\">I consent to the processing of my Personal Data for the purposes detailed under paragraph 2.3 to send information and\/or advertisement material, from the Company or other SOL Group companies, and particularly via e-mail, fax, MMS or SMS messages.<\/span><\/label><\/span><\/span><\/span><\/div>\n<div class=\"col-sm-12\" style=\"font-size:13px; margin-bottom:20px;\"><span class=\"wpcf7-form-control-wrap\" data-name=\"privacy\"><span class=\"wpcf7-form-control wpcf7-acceptance\"><span class=\"wpcf7-list-item\"><label><input type=\"checkbox\" name=\"privacy\" value=\"1\" aria-invalid=\"false\" \/><span class=\"wpcf7-list-item-label\">* I, the undersigned, having read the <a href=\"\/privacy-policy\/\" target=\u201d_blank\u201d title=\"Privacy Policy\"><strong>privacy notice reported here<\/strong><\/a>, which I declare to have read in all its parts, concerning the processing of my personal data required to navigate the Website.<\/span><\/label><\/span><\/span><\/span><\/div>\n<div class=\"col-sm-12\" style=\"margin-top:10px;\"><input type=\"submit\" value=\"Send request\" class=\"wpcf7-form-control has-spinner wpcf7-submit\" \/><\/div>\n<\/div>\n<\/div>\n<div class=\"fusion-alert alert custom alert-custom fusion-alert-center wpcf7-response-output fusion-alert-capitalize alert-dismissable\" role=\"alert\" style=\"border-width:1px;\"><div class=\"fusion-alert-content-wrapper\"><span class=\"fusion-alert-content\"><\/span><\/div><button type=\"button\" class=\"close toggle-alert\" data-dismiss=\"alert\" aria-label=\"Close\">&times;<\/button><\/div><\/form><\/div><\/div><style type=\"text\/css\">.fusion-body .fusion-builder-column-16{width:60% !important;margin-top : 0px;margin-bottom : 0px;}.fusion-builder-column-16 > .fusion-column-wrapper {padding-top : 0px !important;padding-right : 0px !important;margin-right : 3.2%;padding-bottom : 0px !important;padding-left : 0px !important;margin-left : 3.2%;}@media only screen and (max-width:1024px) {.fusion-body .fusion-builder-column-16{width:100% !important;order : 0;}.fusion-builder-column-16 > .fusion-column-wrapper {margin-right : 1.92%;margin-left : 1.92%;}}@media only screen and (max-width:640px) {.fusion-body .fusion-builder-column-16{width:100% !important;order : 0;}.fusion-builder-column-16 > .fusion-column-wrapper {margin-right : 1.92%;margin-left : 1.92%;}}<\/style><\/div><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-17 fusion_builder_column_1_5 1_5 fusion-flex-column\"><div class=\"fusion-column-wrapper fusion-flex-justify-content-flex-start fusion-content-layout-column\" style=\"background-position:left top;background-repeat:no-repeat;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;padding: 0px 0px 0px 0px;\"><\/div><style type=\"text\/css\">.fusion-body .fusion-builder-column-17{width:20% !important;margin-top : 0px;margin-bottom : 0px;}.fusion-builder-column-17 > .fusion-column-wrapper {padding-top : 0px !important;padding-right : 0px !important;margin-right : 9.6%;padding-bottom : 0px !important;padding-left : 0px !important;margin-left : 9.6%;}@media only screen and (max-width:1024px) {.fusion-body .fusion-builder-column-17{width:100% !important;order : 0;}.fusion-builder-column-17 > .fusion-column-wrapper {margin-right : 1.92%;margin-left : 1.92%;}}@media only screen and (max-width:640px) {.fusion-body .fusion-builder-column-17{width:100% !important;order : 0;}.fusion-builder-column-17 > .fusion-column-wrapper {margin-right : 1.92%;margin-left : 1.92%;}}<\/style><\/div><\/div><style type=\"text\/css\">.fusion-body .fusion-flex-container.fusion-builder-row-8{ padding-top : 80px;margin-top : 0px;padding-right : 0px;padding-bottom : 80px;margin-bottom : 0px;padding-left : 0px;}<\/style><\/div><\/p>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":0,"parent":186,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"100-width.php","meta":[],"_links":{"self":[{"href":"https:\/\/www.personalgenomics.it\/en\/wp-json\/wp\/v2\/pages\/1110"}],"collection":[{"href":"https:\/\/www.personalgenomics.it\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.personalgenomics.it\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.personalgenomics.it\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.personalgenomics.it\/en\/wp-json\/wp\/v2\/comments?post=1110"}],"version-history":[{"count":40,"href":"https:\/\/www.personalgenomics.it\/en\/wp-json\/wp\/v2\/pages\/1110\/revisions"}],"predecessor-version":[{"id":2018,"href":"https:\/\/www.personalgenomics.it\/en\/wp-json\/wp\/v2\/pages\/1110\/revisions\/2018"}],"up":[{"embeddable":true,"href":"https:\/\/www.personalgenomics.it\/en\/wp-json\/wp\/v2\/pages\/186"}],"wp:attachment":[{"href":"https:\/\/www.personalgenomics.it\/en\/wp-json\/wp\/v2\/media?parent=1110"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}